Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Iodoacetamide has been used:as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assayin equilibration of pH gradient strips during the second dimension run in isoelectric focusingas a protein alkylation agent

Biochem/physiol Actions

Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).